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Science and Mathematics

Biophysical studies of SNARE-mediated membrane fusion

February 13, 2020 at 3:30pm5:00pm

Physics Building, 202 / 204

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Speaker Jiajie Diao, is an Assistant Prof. at the University of Cincinnati College of Medicine. He is a candidate for a faculty position in the Department of Physics, part of the cluster hiring initiative in the BioInspired Institute. He will be presenting the Physics Department weekly colloquia.

Abstract: Membrane fusion is one of the most important cellular processes by which two initially distinct lipid bilayers merge their hydrophobic cores, resulting in one interconnected structure. The so-called SNARE proteins play a central role in many fusion processes and they regulated by several accessory proteins. In order to study SNARE-mediated membrane fusion, in vitro protein reconstitution assays involving ensemble FRET have been used over a decade. Recently, we have developed single-vesicle-based FRET approaches to overcome shortcomings of ensemble assays for studying the dynamics and kinetics of SNARE-mediated membrane fusion. Moreover, we also created an optogenetic tool to control the conformation of SNAREs and studied the role of protein clusters in membrane fusion.

This event was published on January 27, 2020.


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